Nclex-Rn Practice Questions-Microbiology Practical Applications of Immunology
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Question 1 |
All of the following are generally used in vaccines EXCEPT
A | toxoids. |
B | parts of bacterial cells. |
C | live, attenuated viruses. |
D | inactivated viruses. |
E | antibodies. |
Question 2 |
Haemophilus capsule polysaccharide plus diphtheria toxoid is a(n)
A | inactivated whole-agent vaccine. |
B | attenuated whole-agent vaccine. |
C | conjugated vaccine. |
D | subunit vaccine. |
E | toxoid vaccine. |
Question 3 |
Dead Bordetella pertussis can be used in a(n)
A | inactivated whole-agent vaccine. |
B | attenuated whole-agent vaccine. |
C | conjugated vaccine. |
D | subunit vaccine. |
E | toxoid vaccine. |
Question 4 |
Isolated and purified hepatitis B virus surface antigen can be used in a(n)
A | inactivated whole-agent vaccine. |
B | attenuated whole-agent vaccine. |
C | conjugated vaccine. |
D | subunit vaccine. |
E | toxoid vaccine. |
Question 5 |
In a direct ELISA test to screen for drugs in a patient's urine, what is the third step in the test process?
A | substrate for the enzyme is added |
B | enzyme-labeled antibodies against the drug being tested is added |
C | the patient's urine sample is diluted |
D | antibody against the drug being tested is added |
Question 6 |
Which item is from the patient in a direct ELISA test?
A | substrate for the enzyme |
B | antigen |
C | antihuman immune serum |
D | antibodies against the antigen |
Question 7 |
Which of the following tests is MOST useful in determining the presence of AIDS antibodies?
A | agglutination |
B | complement fixation |
C | neutralization |
D | indirect ELISA |
E | direct fluorescent-antibody |
Question 8 |
Which of the following uses fluorescent-labeled antibodies?
A | agglutination |
B | complement fixation |
C | precipitation |
D | flow cytometry |
E | neutralization |
Question 9 |
Which of the following is NOT an advantage of live attenuated vaccine agents?
A | They elicit lifelong immunity. |
B | They stimulate by cell-mediated and humoral immune responses. |
C | They occasionally revert to virulent forms. |
D | They require few or no booster immunizations. |
E | The immune response generated by the vaccine closely mimics a real infection. |
Question 10 |
In an immunodiffusion test to diagnose the fungal disease histoplasmosis, a patient's serum is placed in a well in an agar plate. In a positive test, a precipitate forms as the serum diffuses from the well and meets material diffusing from a second well. In this test process, what is the most likely identity of the material in the second well?
A | antibodies |
B | a purified fungal antigen |
C | entire fungal cells |
D | a purified protozoan antigen |
E | red blood cells |
Question 11 |
In an immunodiffusion test to diagnose histoplasmosis, a patient's serum is placed in a well in an agar plate. In a positive test, a line forms as the serum diffuses from the well and meets material diffusing from a second well. What type of test is this?
A | an agglutination reaction |
B | a precipitation reaction |
C | a complement-fixation test |
D | an indirect ELISA test |
E | a direct ELISA test |
Question 12 |
Which of the following statements about measles is FALSE?
A | It is a serious disease. |
B | It is preventable by vaccination. |
C | Annually, it kills thousands of children worldwide. |
D | The disease has been eradicated in the United States. |
E | Complications include pneumonia, encephalitis, and death. |
Question 13 |
Live weakened polio virus can be used directly in a(n)
A | inactivated whole-agent vaccine. |
B | attenuated whole-agent vaccine. |
C | conjugated vaccine. |
D | subunit vaccine. |
E | toxoid vaccine. |
Question 14 |
Palivizumab is used to treat respiratory syncytial virus disease. This antiviral drug is a(n)
A | toxoid. |
B | monoclonal antibody. |
C | vaccine. |
D | immunosuppressive. |
E | nucleoside analog. |
Question 15 |
Monoclonal antibodies are used in diagnostic tests and disease treatments because they
A | are highly specific. |
B | can be produced in large quantities. |
C | contain a mixture of antibodies. |
D | are highly specific and they can be produced in large quantities. |
E | are highly specific, they can be produced in large quantities, and they contain a mixture of antibodies. |
Question 16 |
Patient's serum, influenza virus, and red blood cells are mixed in a tube. What happens if the patient has antibodies against influenza virus?
A | agglutination |
B | hemagglutination |
C | complement fixation |
D | hemolysis |
E | hemagglutination-inhibition |
Question 17 |
A patient shows the presence of antibodies against diphtheria toxin. Which of the following statements is FALSE?
A | The patient may have the disease. |
B | The patient may have had the disease and has recovered. |
C | The patient may have been vaccinated. |
D | A recent transfusion may have passively introduced the antibodies. |
E | The patient was near someone who had the disease. |
Question 18 |
Which of the following are sources of antibodies for serological testing?
A | vaccinated animals |
B | cells producing monoclonal antibodies |
C | viral cultures |
D | vaccinated animals and cells producing monoclonal antibodies |
E | vaccinated animals, cells producing monoclonal antibodies, and viral cultures |
Question 19 |
A reaction between an antibody and soluble antigen-forming lattices is called a(n)
A | agglutination reaction. |
B | complement fixation. |
C | immunofluorescence. |
D | neutralization reaction. |
E | precipitation reaction. |
Question 20 |
A reaction between antibody and particulate antigen is called a(n)
A | agglutination reaction. |
B | complement fixation. |
C | immunofluorescence. |
D | neutralization reaction. |
E | precipitation reaction. |
Question 21 |
A reaction that uses the absence of hemolysis of red blood cells to indicate an antigen—antibody reaction is called a(n)
A | agglutination reaction. |
B | complement fixation. |
C | immunofluorescence. |
D | neutralization reaction. |
E | precipitation reaction. |
Question 22 |
A DNA plasmid encoding a protein antigen from West Nile virus is injected into muscle cells of a horse. This is an example of a(n)
A | subunit vaccine. |
B | conjugated vaccine. |
C | nucleic acid vaccine. |
D | attenuated whole-agent vaccine. |
E | live whole-agent vaccine. |
Question 23 |
Toxoid vaccines, such as the vaccines against diphtheria and tetanus, elicit a(n)
A | TC cell response. |
B | immune complex. |
C | dendritic cell proliferation. |
D | antibody response against these bacterial toxins. |
E | antibody response against gram-positive bacteria. |
Question 24 |
The clumping of test red blood cells indicates a negative test result (no antibodies against the virus in the patient's serum) in the
A | direct agglutination test. |
B | indirect agglutination test. |
C | complement-fixation test. |
D | precipitation test. |
E | viral hemagglutination inhibition test. |
Question 25 |
The following steps are used to produce monoclonal antibodies. What is the fourth step?
A | A B cell is activated to produce antibodies. |
B | Culture the hybridoma in a selective medium. |
C | Fuse a B cell to a myeloma cell. |
D | Isolate antibody-producing B cells. |
E | Vaccinate a mouse. |
Question 26 |
Purified protein from Bordetella pertussis is used in a(n)
A | conjugated vaccine. |
B | subunit vaccine. |
C | nucleic acid vaccine. |
D | attenuated whole-agent vaccine. |
E | toxoid vaccine. |
Question 27 |
What type of vaccine involves host synthesis of viral antigens?
A | conjugated vaccine |
B | subunit vaccine |
C | nucleic acid vaccine |
D | attenuated whole-agent vaccine |
E | toxoid vaccine |
Question 28 |
A test used to identify antibodies against Treponema pallidum in a patient's serum is the
A | direct fluorescent-antibody test. |
B | indirect fluorescent-antibody test. |
C | direct agglutination test. |
D | direct ELISA test. |
E | hemagglutination-inhibition test. |
Question 29 |
A test used to identify Streptococcus pyogenes in a patient's throat swab is the
A | direct fluorescent-antibody test. |
B | indirect fluorescent-antibody test. |
C | hemagglutination test. |
D | hemagglutination-inhibition test. |
E | indirect ELISA test. |
Question 30 |
A test used to detect anti-Rickettsia antibodies in a patient's serum is the
A | direct fluorescent-antibody test. |
B | indirect fluorescent-antibody test. |
Question 31 |
Which of the following is a pregnancy test used to find the fetal hormone HCG in a woman's urine using anti-HCG and latex spheres?
A | direct agglutination reaction |
B | indirect agglutination reaction |
C | immunofluorescence |
D | neutralization reaction |
E | precipitation reaction |
Question 32 |
Which of the following is a test to determine a patient's blood type by mixing the patient's red blood cells with antisera?
A | direct agglutination reaction |
B | passive agglutination reaction |
C | immunofluorescence |
D | neutralization reaction |
E | precipitation reaction |
Question 33 |
Which of the following is a test to determine the presence of soluble antigens in a patient's saliva?
A | direct agglutination reaction |
B | passive agglutination reaction |
C | immunofluorescence |
D | neutralization reaction |
E | precipitation reaction |
Question 34 |
A patient's serum, Mycobacterium, guinea pig complement, sheep red blood cells, and anti-sheep red blood cell antibodies are mixed in a test tube. What happens if the patient has antibodies to Mycobacterium?
A | Bacteria fluoresce. |
B | Hemagglutination occurs. |
C | Hemagglutination-inhibition occurs. |
D | Hemolysis occurs. |
E | No hemolysis occurs. |
Question 35 |
A vaccine against HIV proteins made by a genetically-engineered vaccinia virus that has infected a eukaryotic cell line is a(n)
A | conjugated vaccine. |
B | subunit vaccine. |
C | nucleic acid vaccine. |
D | inactivated whole-agent vaccine. |
E | toxoid vaccine. |
Question 36 |
Inactivated tetanus toxin is a(n)
A | conjugated vaccine. |
B | subunit vaccine. |
C | nucleic acid vaccine. |
D | inactivated whole-agent vaccine. |
E | toxoid vaccine. |
Question 37 |
A hybridoma results from the fusion of a(an)
A | B cell with a T cell. |
B | B cell with a myeloma cell. |
C | antigen with an antibody. |
D | antigen with a B cell. |
E | myeloma cell with a virus. |
Question 38 |
What type of vaccine is the live, weakened measles virus?
A | conjugated vaccine |
B | subunit vaccine |
C | nucleic acid vaccine |
D | attenuated whole-agent vaccine |
E | toxoid vaccine |
Question 39 |
In an agglutination test, eight serial dilutions to determine antibody titer were set up. Tube #1 contained a 1:2 dilution; tube #2, a 1:4, etc. If tube #6 is the last tube showing agglutination, what is the antibody titer?
A | 6 |
B | 1:6 |
C | 64 |
D | 1:32 |
E | 32 |
Question 40 |
An ELISA for Hepatitis C has 95 percent sensitivity and 90 percent specificity. This means that the test
A | detects 95 percent of the true positive samples and has 10 percent false positive results. |
B | detects 5 percent of the true positive samples and has 90 percent false positive results. |
C | detects 90 percent of the true positive samples and has 5 percent false positive results. |
D | detects 95 percent of the true positive samples and has 90 percent false positive results. |
E | detects 5 percent of the true positive samples and has 10 percent false positive results. |
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