Nclex-Rn Practice Questions-Microbiology Microbial Growth
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Question 1 |
A culture medium consisting of agar, peptone, and beef heart is a
A | chemically defined medium. |
B | complex medium. |
C | selective medium. |
D | differential medium. |
E | reducing medium. |
Question 2 |
The source of nutrients in nutrient agar is
A | agar. |
B | gelatin. |
C | peptone and beef extract. |
D | peptone and NaCl. |
E | agar and NaCl. |
Question 3 |
Which of the following is an example of a metabolic activity that could be used to measure microbial growth?
A | standard plate count |
B | glucose consumption |
C | direct microscopic count |
D | turbidity |
E | most probable number (MPN) |
Question 4 |
Which group of microorganisms is most likely to spoil a freshwater trout preserved with salt?
A | psychrophiles |
B | facultative halophiles |
C | anaerobes |
D | thermophiles |
E | hyperthermophiles |
Question 5 |
Which of the following is NOT a direct method to measure microbial growth?
A | direct microscopic count |
B | standard plate count |
C | filtration on a support membrane followed by incubation on medium |
D | metabolic activity |
E | most probable number (MPN) |
Question 6 |
Which of the following is the best definition of generation time?
A | the length of time needed for lag phase |
B | the length of time needed for a cell to divide |
C | the minimum rate of doubling |
D | the duration of log phase |
E | the time needed for nuclear division |
Question 7 |
During which growth phase will gram-positive bacteria be most susceptible to penicillin?
A | lag phase |
B | log phase |
C | death phase |
D | stationary phase |
E | The culture is equally susceptible during all phases. |
Question 8 |
Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented a sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n)
A | selective medium. |
B | differential medium. |
C | enrichment culture. |
D | selective and differential medium. |
E | differential and enrichment culture. |
Question 9 |
Most bacteria reproduce by
A | aerial hyphae. |
B | fragmentation. |
C | mitosis. |
D | binary fission. |
E | budding. |
Question 10 |
Which of the following is an advantage of the direct microscopic count?
A | can readily count organisms that are motile |
B | can easily distinguish live from dead cells |
C | requires no incubation time |
D | sample volume is unknown |
E | requires a large number of cells |
Question 11 |
Patients with indwelling catheters (long-term tubes inserted into body orifices for drainage, such as through the urethra and into the urinary bladder) are susceptible to infections because
A | injected solutions are contaminated. |
B | their immune systems are weakened. |
C | infections can be transmitted from other people. |
D | biofilms develop on catheters. |
E | bacteria cause infections. |
Question 12 |
The term aerotolerant anaerobe refers to an organism that
A | does not use oxygen but tolerates it. |
B | is killed by oxygen. |
C | tolerates normal atmospheric nitrogen gas levels. |
D | requires less oxygen than is present in air. |
E | requires more oxygen than is present in air. |
Question 13 |
Which of the following elements is NOT correctly matched with its cellular function?
A | nitrogen — needed for amino acid synthesis |
B | phosphorus — incorporated into nucleic acids |
C | sulfur — used for synthesis of thiamin and biotin |
D | magnesium and potassium — required as cofactors for enzymes |
E | phosphorus — used for production of carbohydrates. |
Question 14 |
Pathogenic bacteria isolated from the respiratory or intestinal tracts of humans are
A | strict aerobes that grow best in candle jars. |
B | capnophiles that grow best in carbon dioxide incubators. |
C | facultative anaerobes that require reducing media for growth. |
D | strict aerobes that grow best in reducing media. |
E | capnophiles that prefer highly oxygenated growth conditions. |
Question 15 |
Which of the following is an advantage of the standard plate count?
A | can readily count cells that form aggregates |
B | determines the number of viable cells |
C | can be performed on very dilute samples, such as lake water |
D | provides immediate results |
E | can be used to count heat-sensitive bacteria |
Question 16 |
Salts and sugars work to preserve foods by creating a
A | depletion of nutrients. |
B | hypotonic environment. |
C | lower osmotic pressure. |
D | hypertonic environment. |
E | lower pH. |
Question 17 |
The addition of which of the following to a culture medium will neutralize acids?
A | buffers |
B | sugars |
C | pH |
D | heat |
E | carbon |
Question 18 |
The biosafety level (BSL) for a clinical microbiology laboratory working with potentially airborne pathogens, such as tuberculosis bacteria, is
A | BSL-1. |
B | BSL-2. |
C | BSL-3. |
D | BSL-4. |
Question 19 |
The biosafety level (BSL) for most introductory microbiology laboratories is
A | BSL-1. |
B | BSL-2. |
C | BSL-3. |
D | BSL-4. |
Question 20 |
Which of the following pairs of microbe classification terms and optimal growth temperatures is mismatched?
A | psychrotroph — growth at 0°C |
B | thermophile — growth at 37°C |
C | mesophile — growth at 25°C |
D | psychrophile — growth at 15°C |
E | hyperthermophiles — growth at 85°C |
Question 21 |
A sample of milk is tested for its bacterial content in a plate count assay. A one-milliliter sample of the milk is diluted in a 1:10 dilution series. One milliliter of the third dilution tube is plated in a pour plate. After incubation, the plate has 54 colonies, indicating that the original milk sample contained
A | 54 cells per milliliter. |
B | 540 cells per milliliter. |
C | 5,400 cells per milliliter. |
D | 54,000 cells per milliliter. |
E | 540,000 cells per milliliter. |
Question 22 |
Most fungi grow best at pH
A | 1. |
B | 5. |
C | 7. |
D | 9. |
E | 14. |
Question 23 |
Which of the following is an organic growth factor?
A | glucose |
B | vitamin B1 |
C | peptone |
D | Mg+2 |
E | H2O |
Question 24 |
Most bacteria grow best at pH
A | 1. |
B | 5. |
C | 7. |
D | 9. |
E | 14. |
Question 25 |
Which enzyme catalyzes the reaction: H2O2 + 2H+ → 2H2O?
A | catalase |
B | oxidase |
C | peroxidase |
D | superoxide dismutase |
Question 26 |
Which enzyme catalyzes the reaction: O2- + O2- + 2H+ → H2O2 + O2?
A | catalase |
B | oxidase |
C | peroxidase |
D | superoxide dismutase |
Question 27 |
Which enzyme catalyzes the reaction: 2H2O2 → 2H2O + O2?
A | catalase |
B | oxidase |
C | peroxidase |
D | superoxide dismutase |
Question 28 |
An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through?
A | 64 |
B | 32 |
C | 6 |
D | 5 |
E | 4 |
Question 29 |
In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary tract of 10 percent of 1300 patients who underwent gastrointestinal endoscopic procedures. After each use, endoscopes were washed with an automatic reprocessor that flushed detergent and glutaraldehyde through the endoscopes, followed by a tap water rinse. P. aeruginosa serotype 10 was not isolated from the detergent, glutaraldehyde, or tap water. What was the source of the infections?
A | bacterial cell walls in the water |
B | a biofilm in the reprocessor |
C | contaminated disinfectant |
D | fecal contamination of the bile ducts |
E | None of the answers is correct. |
Question 30 |
Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count. How many cells were in the original sample?
A | 4 per milliliter |
B | 9 per milliliter |
C | 18 per milliliter |
D | 36 per milliliter |
E | 72 per milliliter |
Question 31 |
If cells are grown in media containing amino acids labeled with radioactive nitrogen (15N), most of the radioactivity will be found in the cells'
A | DNA. |
B | proteins. |
C | phospholipids. |
D | DNA and proteins. |
E | DNA and phospholipids. |
Question 32 |
Three cells with generation times of 60 minutes are inoculated into a culture medium. How many cells are there after 5 hours?
A | 900 |
B | 180 |
C | 96 |
D | 32 |
E | 15 |
Question 33 |
Assume you inoculated 100 cells, with a generation time of 20 minutes, into 100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml of nutrient broth. After incubation for 4 hours, you can reasonably expect to have
A | more cells in the 100 ml. |
B | more cells in the 200 ml. |
C | the same number of cells in both. |
D | The answer cannot be determined based on the information provided. |
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